Product
제품
- GeneJect™
- 제품명: GeneJect™
- 용도: DNA, siRNA / miRNA transfection reagent
- 메이커: A&A Biotechnology
- 카달로그:
소개
GeneJect™
DNA, siRNA / miRNA transfection reagent
• Low cell toxicity
• Efficient transfection of wide variety of primary cells and cell lines
Description
GeneJect™ is a transfection reagent, which is suitable for the delivery of plasmid DNA and short oligonucleotides, such as siRNAs as well as miRNA mimics and inhibitors.
GeneJect™ is less toxic when compared with lipofection. Toxicity is higher at low cell confluency.
GeneJect™ is efficient in the transfection of common cell lines of different origin, including HeLa, HEK293, PPC-1, HaCaT, A549, BEAS-2B, CCD19Lu, THP-1 and KOP (fetal bovine oropharynx cells). It also enables transfection of low level transfection cells including MDBK (Madin Darby Bovine Kidney cells).
GeneJect™ is efficient in the transfection of wide variety of primary cells, such as human primary keratinocytes, primary skin fibroblasts, melanocytes and monocytes.
100 μl of GeneJect™ allows for up to 50 transfections in 12-well tissue culture plates format.
DNA Transfection performance of GeneJect™ versus market top-standard transfection reagent
Figure 1. Uptake of GeneJect™ – plasmid DNA nanocomplexes
Cells were transfected with pEGFP plasmid DNA on a 12 well plate with 1 μg DNA and 2 μl Gene Ject™ per well and visualised under fluorescence microscope after 24h from transfection.
| HEK | MDBK | |
| GeneJect™ |  |
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| jetPRIME® |  |
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HEK - Easy to transfect Human embryonic kidney cells.
MDBK - Hard to transfect Madin Darby bovine kidney cells.
miRNA Transfection performance of GeneJect™ versus market top-standard transfection reagent
Figure 2. Uptake of GeneJect™ – miRNA nanocomplexes
Dy547-labelled (red) miRNA mimics were transfected with either GeneJect™ or with a lipofectamine type of reagent siPORT-NeoFX at 30 nM concentration and stained after 24 h with DAPI (blue) and Alexa Fluor A488 Phalloidin (green).
| A549 | BEAS-2B | CCD19-Lu | PPC-1 | |
| GeneJect™ |  |
 |
 |
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| siPORT |  |
 |
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| Dy547 Phalloidin DAPI | ||||
A549 - Adenocarcinomic human alveolar basal epithelial cells,
BEAS-2B - Human bronchial epithelial cell line,
CCD19-Lu - Human lung fibroblast cells,
PPC-1 - human prostatic cell line.
siRNA Transfection performance of GeneJect™
Figure 3. Inhibition of target gene (IRAK1) by GeneJect™ delivered miR-146a mimic
Cells were transfected either with control (cont) or miR-146a mimics at 30 nM concentration. After 48 hours, a total RNA was purified and analyzed by RT-qPCR using primers specific to IRAK1. Relative expression compared to cont in each cell type (=1) is shown. Data represent mean ± SEM of four independent transfection. Student’s t-test, ** P < 0.01.
PPC-1 - Human prostatic cell line,
A549 - Adenocarcinomic human alveolar basal epithelial cells,
KC - Human primary keratinocytes,
THP-1 - Human monocytic cell-line.
Figure 4. GeneJect™ efficiency in siRNA delivery
Human primary keratinocytes were transfected either with control (cont) or indicated specific siRNAs at 30 nM concentration using GeneJect™. After 48 hours, a total RNA was purified and analyzed by RT-qPCR using primers specific to IRAK1 and CARD10. Relative expression compared to cont siRNA (=1) is shown. Data represent mean ± SEM of three independent transfection.
Student’s t-test, * P < 0.05. ** P < 0.01.
주문정보
4100-01 GeneJect™ 100 µl
4100-05 GeneJect™ 500 µl
4100-15 GeneJect™ 1.5 ml
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