Product
소개
JO-FI™ competent cells
High efficiency competent cells
High efficiency competent cells suitable for any type of cloning method and standard plasmid DNA transformation. Stable replication of high-copy plasmids.
1. Advantages of the product
High transformation efficiency: 5–6 x 10⁹ cfu/µg pUC19 DNA
2. Description
JO-FI™ competent cells are derived from the E. coli DH5α. The strain is not pathogenic and has been developed specifically for molecular cloning (Biohazard level 1).
3. Comparison of transformation efficiency
Transformation with a high-copy PUC19 plasmid (1 ng) and 50µl of competent cells.
JO-FI™ | TOP 10 f' |
Ligation mixture contained as follows: The pUC19 vector digested with restrictases at the lacZ gene; The insert fragment with compatible DNA ends digested with the same restrictases as vector molecule. Both DNA frafments were mixed at the vector to insert molar ratio of 1:3 and subsequent ligation / transformation performed accordingly to standard procedure. The blue/white recombinant selection was performed by speading out and cultivation of ligation mixture with competent cells on agar media supplemented with X-Gal and IPTG.
3-2. Cohesive ends
JO-FI™ | TOP 10 f' |
3-3. Blunt ends
JO-FI™ | TOP 10 f' |
사양
• The lacIq repressor enables tight, IPTG -dependent expression control from the trc, tac and lac promoters.
• The lacZΔM15 deletion allows the white-blue color selection of recombinant clones (LA + X-Gal).
• recA1 gene mutation reduces the occurrence of undesired recombination events during cloning.
• endA1 gene mutation decreases endonuclease I activity. This results in a lower level of DNA degradation,
providing faster plasmid DNA transfer.
• mcrA gene mutation allows efficient transformation of methylated genomic DNA.
• hsdR gene mutation allows efficient transformation of unmethylated DNA after PCR amplification.
주문정보
1033-20 JO-FI™ competent cells 20 pcs
1033-20 JO-FI™ competent cells 40 pcs
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