Product
소개
Fluoro MPOHOCL
Myeloperoxidase Chlorination and peroxidation Detection Kit (Fluorescence)
1. Key Benefits
• Can monitor multiple time points to follow kinetics.
• One-step, no wash assay.
• Adaptable for High Throughput format.
• Highly Sensitive.
• Applications – Fluorescent Plate Reader.
2. Assay Principle
Myeloperoxidase (MPO) is a highly cationic glycosolated hemoprotein that has a molecular weight of 144kD. The hemoprotein consists of two dimers linked via a disulfide bridge. Each dimmer is composed of a heavy (53kD) and light (15kD) subunit. Each heavy chain contains an independently acting protoporphyrin group containing a central iron (1-5). MPO is present in the azurophilic granules of polymorphonuclear leukocytes (PMNs) and is unique to neutrophils and monocytes. However, monocytes contain only one third of the MPO found in PMN’s. MPO utilizes H202 produced by the neutrophils to oxidize a varity of aromatic compounds to give substrate radicals for bactericidal activity (4 review). This enzyme is unique however in that it can oxidize chloride ions to produce a strong nonradical oxidant,HOCl. HOCl is the most powerful bactericidal produced by neutrophils (4 review). Excessive production of these radicals can cause oxidative stress leading to oxidative tissue injury.
Chlorination Reaction:
H2O2 + MPO + Cl- —-> HOCl + APF (non fluorescent) —-> Fluorescent Dye
Excitation:488nm; Emission: 515-530nm
Peroxidation Reaction:
H2O2 + Detection reagent (non-fluorescent)+ MPO —–> fluorescent analog
Excitation 530-571nm Emission 590-600nm
주문정보
Reagent |
Catalog # |
Size |
Chlorination and Oxidation Detection Kit |
MPOHOCL100-3 |
500 + 300 tests |
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