Product
소개
SELECTRAZYME β-Glucuronidase
Abalone derived β-glucuronidase is used for the enzymatic hydrolysis of glucuronides from urine, blood and serum prior to analysis by enzyme immunoassay, mass spectrometry, high performance liquid chromatography, and other means. Typically, between 1 to 10 units of glucuronidase is used per microliter of the sample matrix. The exact amount needed will depend on the specific conditions used and must be determined empirically.
Abalone derived β-glucuronidase is a crude solution of enzymes. Many β-glucuronidases derived from mollusks also contain sulfatase activity. For this reason, the sulfatase activity of the material is also determined. Abalone derived β-glucuronidase is more thermal tolerant as compared to enzymes derived from E. coli, H. pomatia and bovine liver. Therefore the hydrolysis reaction can be carried out at a higher temperature providing hydrolysis in less time and achieving a higher degree of hydrolysis of metabolites like morphine-3-glucuronide.
Another application of Abalone derived β-glucuronidase is for the deconjugation of steroids in human and animal urine samples. When compared with H. pomatia, Limpet and bovine liver, abalone derived β-glucuronidase provides best results. For reference to this study, please site "Enzymatic Hydrolysis of Conjugated Steroid Metabolites: Search for Optimum Conditions Using Response Surface Methodology" V. Ferchaud, P. Courcoux, B. LeBizec, F. Monteau & F. Andre, The Analyst, 2000, 125, 2255-2259.
주문정보
Part Number | Description | Size | Units |
---|---|---|---|
BETA-GLUC-10 |
Beta Glucuronidase Enzyme (abalone) - liquid form |
10 mL
|
1
|
BETA-GLUC-25 |
Beta Glucuronidase Enzyme (abalone) liquid form |
25 mL
|
1
|
BETA-GLUC-50 |
Beta Glucuronidase Enzyme (abalone) liquid form |
50 mL
|
1
|
BETA-GLUC-100 |
Beta Glucuronidase Enzyme (abalone) liquid form |
100 mL
|
1
|
BETA-GLUC-250KU |
Beta-Glucuronidase Enzyme (abalone) lyophilized 250,000 fishman units |
250,000 units
|
1
|
BETA-GLUC-500KU |
Beta-Glucuronidase Enzyme (abalone) lyophilized 500,000 fishman units |
500,000 units
|
1
|
BETA-GLUC-1MU |
Beta-Glucuronidase Enzyme (abalone) lyophilized 1,000,000 fishman units |
1,000,000 units
|
1
|
BETA-GLUC-2MU |
Beta-Glucuronidase Enzyme (abalone) lyophilized 2,000,000 fishman units |
2,000,000 units
|
1
|
관련자료
Evaluation of UCT's Selectrazyme for Forensic Applications
Determination of Anabolic Steroids in Horse Urine by SPE and LC-MS/MS
Quantitative Analysis of Anabolic Steroids in Urine using Mixed-Mode SPE and LC-MS/MS