Product
소개
Lipid Peroxidation – LPO Assay Kit
소개 (Description)
Lipid peroxidation is a well-known example of oxidative damage in cell
membranes, lipoproteins, and other lipid-containing structures. Peroxidative
modification of unsaturated phospholipids, glycolipids, and cholesterol can occur
in different reactions. They can be triggered by i) free radical species such as
oxyl radicals, peroxyl radicals, and hydroxyl radicals derived from iron-mediated
reduction of hydrogen peroxide or ii) non-radical species such as singlet oxygen,
ozone, and peroxynitrite generated by the reaction of superoxide with nitric oxide.
Malondialdehyde (MDA) and 4-hydroxyalkenals are important toxic byproducts of
lipid peroxidation. So, the measurement of the amounts of such aldehydes
corresponds to an index of lipid peroxidation in vitro and in vivo.
4-Hydroxynonenal(4-HNE) is a major product of the peroxidative decomposition
of ω-6 polyunsaturated fatty acids (PUFA). It possesses cytotoxic, hepatotoxic,
mutagenic, and genotoxic properties. Furthermore, increased levels of HNE were
found in plasma and various organs under oxidative stress conditions. In fact, MDA
is in many instances the most abundant individual aldehyde resulting from lipid peroxidation.
In vitro MDA can alter proteins, DNA, RNA, and many other biomolecules.
분석 원리 (Assay Principles)
Bioquochem’s LPO assay kit measures MDA and HNE concentrations as an index of lipid peroxidation. Firstly, acid-catalyzed attack at the 3-position of the indole ring initiates the reactions between indoles and aldehydes (MDA and HNE). As a result, this reaction gives a diindolylalkane (chromophore) with maximum absorbance in the region of 580-620 nm.
In our assay an indol (Reagent A) reacts quickly with MDA and HNE in acidic medium, yielding a chromophore (C) with a high molar extinction coefficient at its maximal absorption wavelength of 586 nm.
Reagent A + MDA → C (λmáx = 586 nm)
Reagent A + HNE → C (λmáx = 586 nm)
Scheme 1. Reactions between aldehydes and indoles
주문정보
KB03002-200 Lipid Peroxidation – LPO Assay Kit 200 tests
KB03002-400 Lipid Peroxidation – LPO Assay Kit 400 tests
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