CUPRAC Antioxidant Capacity Assay Kit

소개 (Description)

CUPRAC (CUPric Reducing Antioxidant Capacity) is a mostly new method which, for example, enables TAC measures even in oils. The main reagent, the copper (II)-neocuproin (2,9-dimethyl-1,10-phenanthroline), can oxidize antioxidants generating a coloured product measurable by colorimetric methods. The chelation with neocuproin enables a fastened reaction by elevating the redox potential of the reagent.

Recent correlations have been saw within many illnesses and ROS (reactive species of oxygen, as superoxide); antioxidants on the other hand have been proved to inhibit this ROS. So the measure of these antioxidants seems an interesting new approach to study not only illnesses, also many other physiological states. 

분석 원리 (Assay Principle)

CUPRAC reagent implies an oxidation of an antioxidant with a leading tiol group, like for example gluthation (GSH). In this process, the reagent reduces itself forming a chelate complex of cupper (I) – neocuproin, wich provides color measurable at 450nm in a spectrophotometer. This reaction takes place on physiological pH (or neutral pH, near pH 7).

For agrifood samples and biological samples with deproteinization step 

KF01005-100      CUPRAC Antioxidant Capacity Assay Kit for Biological Samples      100 test

KF01005-200      CUPRAC Antioxidant Capacity Assay Kit for Biological Samples      200 test

KF01005-400      CUPRAC Antioxidant Capacity Assay Kit for Biological Samples      400 test

For biological samples without deproteinization step – direct measurement

KF01005B-200      CUPRAC Antioxidant Capacity Assay Kit for Biological Samples      200 test

KF01005B-400      CUPRAC Antioxidant Capacity Assay Kit for Biological Samples      400 test

Manual_KF01005 

Manual_KF01005B

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