Peroxide Quantification Assay Kit

소개 (Description)

Reactive oxygen (ROS) and nitrogen (RNS) species are produced in metabolic and physiological processes and can be overproduced in response to different stimuli. Organisms have different enzymatic and nonenzymatic antioxidative mechanisms to ameliorate the harmful effects of these oxidant species.

An imbalance between oxidant species and the antioxidant defense system leads to an oxidative stress state condition that causes damage to cellular biomolecules, and it is involved in the development of several diseases (e.g. cancer, Parkinson, Alzheimer). Oxidative stress can be evaluated directly by measuring ROS. Major ROS include superoxide, hydroxyl radicals and peroxides.

분석 원리 (Assay Principles)

BQC Peroxide Assay Kit is based on the chromogenic Fe 3+ -xylenol orange reaction. Peroxides present in the sample oxidizes ferrous ion (Fe 2+ ) to ferric ion (Fe 3+ ). In an acidic medium the generated Fe 3+ complexes with xylenol orange dye to yield a colored product with maximum absorbance at 580 nm. The amount of complex generated is proportional to the total peroxide concentration in the sample. In this kit H2O2 is used as standard and results are expressed as H2O2 equivalents (µM H2O2).

KB03038-200      Peroxide Quantification Assay Kit      200 tests

KB03038-400      Peroxide Quantification Assay Kit      400 tests 

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