Total Nitric Oxide Assay Kit

 

 

소개 (Description)

 

Nitric oxide (NO) is a product of the conversion of L-arginine by nitric oxide synthase. NO plays an important role in many key physiological and pathological processes such as neurotransmission, immune response and apoptosis.

 

Although NO’s action in the biological system is very versatile, the mostimportant role of NO is the activation of guanylate cyclase. The binding of NO to the heme region of the enzyme in the presence of iron leads to activation, and diffuses freely across membranes as a transient paracrine and autocrine signaling molecule. Once NO is converted to nitrates and nitrites by oxygen and water, cell signaling is deactivated. 

 

BQC Nitric Oxide Assay Kit is a quick, easy and reproducible assay to quantify number in a wide variety of samples. 

 

 

 

 

 

분석 원리 (Assay Principles)

BQC Nitric Oxide Assay Kit is designed to accurately measure NO production following reduction of nitrate to nitrite by using an improved Griess method.

 

The assay’s mechanism is based in the azo coupling between diazonium species, which are produced from the interaction between sulfanilamide and NO 2 - with N-(1-naphthyl) ethylenediamide dihydrochloride resulting in a colorimetric product. Absorbance measured at 540 nm is directly proportional to the concentration of NO in the sample.

 

 

 

 

 

KB03046-100      Total Nitric Oxide Assay Kit      100 tests

KB03046-200      Total Nitric Oxide Assay Kit      200 tests

KB03046-400      Total Nitric Oxide Assay Kit      400 tests 

 

 

 

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