Ascorbate Peroxidase (APX) Assay Kit

 

 

소개 (Description)

Ascorbate peroxidase (APX: EC 1.11.1.11) is a Class I heme-containing peroxidase specific to plants and algae that is indispensable to protect chloroplasts and other cell constituents from reactive oxygen species (ROS) damage.

 

APX is a central component of the ascorbate-glutathione (AsA-GSH) cycle that is one of the most important components of plant antioxidant defense.

 

APX catalyzes the conversion of H2O2 to H2O using ascorbate as the specific electron donor, with the concomitant generation of monodehydroascorbate (MDHA).

 

APX exists as several isoforms that are found in distinct subcellular compartments, such as chloroplasts, mitochondria, peroxisome, and cytosol. The expression of APX genes is regulated in response to biotic and abiotic stresses as well as during plant development. APX activity similar to other antioxidant enzymes varies in response to environmental stress. Measuring APX activity can be therefore considered as indicator of plant redox status.

 

BQC APX Activity Assay Kit is an easy and fast assay to determine APX activity in plants.

 

 

분석 원리 (Assay Principles)

APX catalyzes the conversion of H2O2 to H2O using ascorbate as the specific electron donor. In this APX assay kit, APX activity is determined by the rate of ascorbate oxidation at 290 nm.

 

 

KB03036-100      Ascorbate Peroxidase (APX) Assay Kit      100 tests

KB03036-200      Ascorbate Peroxidase (APX) Assay Kit      200 tests

KB03036-400      Ascorbate Peroxidase (APX) Assay Kit      400 tests 

 

 

 

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