NADP/NADPH Quantification Assay Kit

소개 (Description)

Nicotinamide adenine dinucleotide phosphate NADP(H) is a well-known coenzyme found in all living cells that plays an important role in energy metabolism, biosynthesis and detoxification. NADP(H) exists in cells in oxidized (NADP + ) and reduced (NADPH) forms reflecting the redox state of the cell. Prolonged disequilibrium of NADP + /NADPH balance disturbs the physiological functions, resulting in several diseases like diabetes, cardiovascular diseases, neurodegeneration disorders or cancer.

BQC NADP + /NADPH Assay Kit is a quick, easy, and reproducible assay for determining NADP + /NADPH concentration in cells and tissues.

분석 원리 (Assay Principles)

The BQC NADP + /NADPH Assay Kit is based on an enzymatic cycling reaction in which NADP + is reduced to NADPH. The NADPH present in the sample and the newly formed NADPH are then oxidized by an electron intermediate acceptor resulting in the reduction of a tetrazolium salt to formazan. Formazan is a highly colored compound that shows a maximum absorption at 570 nm. The amount of formazan generated is proportional to the total amount of NADP(H) (NADP + + NADPH) in the sample.

The native NADPH can be determined alone by heating the sample (thermal degradation of NADP + ) before the assay.

KB03034-200      NADP/NADPH Quantification Assay Kit      200 tests

KB03034-400      NADP/NADPH Quantification Assay Kit      400 tests 

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