LEctPROFILE Kits

Evaluation of crude or purified glycoconjugates interactions

 

 

소개(Description)

The LEctPROFILE® kit allows efficient evaluation of crude or purified glycoconjugates interactions (i.e. synthetic molecules or glycoconjugates) with lectins by a simple measurement of absorbance or fluorescence. The LEctPROFILE® kit enables a fast measurement (below 3 h) and are easily accessible to all.

 

Each kit is composed of a 96-well plate immobilised with the target lectin, and supplemented with the corresponding tracor and the revealing solution.

 

All our lectins are controlled under a standardized procedure assessed by interactions with specific neoglycoproteins or glycoproteins through GLYcoPROFILE® method.

 

LEctPROFILE® kits are for research use only.

 

 

 

어플리케이션(Applications)

LEctPROFILE® kits are designed for relevant applications such as:

 

1) Detection of desirable or undesirable glycans during the early stage of quality control of biotherapeutics;

2) Identification of glyco-biomarkers related to cells behaviour and/or pathology;

3) Analysis and characterisation of the glycosylation level of glycoproteins contained in biological fluids or to target the sialylation status of relevant serum biomarkers;

4) Research of antagonist for lectins of interest that are well-known to be involved in particular disorder or in biological mechanism.

 

 

 

LEctPROFILE® kits 사용방법

 

The evaluation of compounds interactions with lectins is possible by two different modes : 

 

1. Competition Mode : 

 

Competitive interaction between a sample and a specific labelled tracer know to have good affinity for the lectin without preliminary labelling of sample. This mode is typically used for the evaluation of the IC50- value (concentration corresponding to 50% of lection inhibition), for the screening of potential candidates, for avidity comparison or for batch to batch monitoring. 

 

 

 

2. Direct Binding 

 

to evaluate potential interaction of compounds ranging from pure molecule to complex mixtures ( glycocojugates(s), complex carbohydrates pr glycomometics). Previous labeling of target molecule(s) by biotinylation or by fluoresceinylation is required for readout. 

 

 

 

 

 

 

LEctPROFILE® kits 제품 리스트

 

 

 

 

 

 

FimH LEctPROFILE® kit

DC-SIGN & Langerin LEctPROFILE® kit

Galectin-3 LEctPROFILE® kit
Cells LEctPROFILE® kit

α2.3_α2.6 LEctPROFILE® kit

Galili LEctPROFILE® kit