Product

제품

HemogloBind™: 제품명: HemogloBind™; 용도: Hemoglobin Removal Kits; 메이커: Biotech Support Group; 카달로그:

소개
사양
주문정보
관련자료

소개

HemogloBind™

혈액 및 조직 유래 시료용 헤모글로빈 제거·포획 키트

HemogloBind™ 는 헤모글로빈에 대한 높은 특이성과 낮은 교차반응성을 바탕으로, 다양한 혈액 및 조직 율는 실리카 기반의 단백질 농축 매트릭스로, 적혈구 용해물 시료에서 헤모글로빈을 제거하는 동시에 저농도/ 저분자량 단백질을 농축할 수 있도록 설계된 제품입니다. 본 제품은 완화된 버퍼 조건을 사용하므로, 용출 분획 내 native 효소 활성이 유지됩니다.

개별 mixed-mode ligand 조합 (이온성, 소수성, 방향족, 고분자 기반)으로 구성된 실리카 기반 라이브러리에서 유래하였습니다. 이 라이브러리는 단백질과의 약한 결합을 유도하도록 설계되어, 출발 시료 내 단백질 구성을 미리 알지 못하더라도 매트릭스로부터 신속한 용출이 가능하도록 합니다. 그 결과 적혈구 용해물에서 헤모글로빈을 제거하는 동시에, 저농도 혈액 단백질의 분리 해상도를 향상시킵니다.

제품 특징

• 헤모글로빈에 높은 특이성
• 다른 단백질과의 교차반응 최소화
• 용혈 시료, 전혈, DBS, 적혈구 용해물, 조직 균질액에 적용 가능
• 분석 간섭 제거 및 프로테오믹스 분석에 적합
• 종 특이성 없음

실험 과정

단계	용혈 혈청 Microfuge Tube	용혈 혈청 Spin-Filter Tube	전혈 Microfuge Tube	전혈 Spin-Filter Tube
1. 시료 준비	용혈 혈청 250 μL (약 10 mg Hb/mL)	용혈 혈청 250 μL (약 10 mg Hb/mL)	전혈 10–20 μL + 0.02 M K₂HPO₄ (pH 6.5) 100–200 μL, 5분 vortex	전혈 10–20 μL + 0.02 M K₂HPO₄ (pH 6.5) 100–200 μL, 5분 vortex
2. HemogloBind™ 첨가	250 또는 500 μL	250 또는 500 μL	100–200 μL	100–200 μL
3. 혼합	30초 vortex 후 실온 10분 반응	20초 vortex 후 실온 10분 반응	20초 vortex 후 실온 10분 반응	20초 vortex 후 실온 10분 반응
4. 원심분리	9,000 rpm (약 8,000 × g), 1–2분	9,000 rpm (약 8,000 × g), 1–2분	9,000 rpm (약 8,000 × g), 4분	9,000 rpm (약 8,000 × g), 1–2분
5. 분획 회수	상등액(supernatant): 헤모글로빈 제거 시료 Pellet / Matrix: 제거된 헤모글로빈	여과액(filtrate): 헤모글로빈 제거 시료 필터 잔류물: 제거된 헤모글로빈	상등액(supernatant): 헤모글로빈 제거 시료 Pellet / Matrix: 제거된 헤모글로빈	여과액(filtrate): 헤모글로빈 제거 시료 필터 잔류물: 제거된 헤모글로빈

HemogloBind™ 시료 부피 비율

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이 도표는 HemogloBind™ 와 시료 간의 적절한 부피 비율을 설정하기 위한 가이드입니다. 용혈 적혈구처럼 대부분 헤모글로빈으로 구성된 시료나, 용혈된 혈청처럼 대부분 혈청/혈장 단백질을 포함하는 시료에서 헤모글로빈 양을 추정하는 데 사용할 수 있습니다. 연구 목적에 따라 최적화를 위해 부피 비율을 조정하시기 바랍니다.

☞ HemogloBind™ Product Sheet 보기

☞ HemogloBind™ Hemoglobin Depletion Performance On Blood Sheet 보기

☞ HemogloBind™ Hemoglobin Depletion Performance On Dried Blood Spots/Cards 보기

사양

HemogloBind_사양.PNG

주문정보

화면 캡처 2026-05-19 104417.png

관련자료

References

Hemolyzed Serum

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Reticulocytes

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Hemocyte fractions

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Perfusates

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Hemolysate

Dasauni, Pushpanjali, et al. "Refractive index of blood is a potential qualitative indicator of hemoglobin disorder in humans." Journal of Proteins & Proteomics 9.3 (2018).

Cell lysates of reticulocytes

Junqueira, Caroline, et al. "Cytotoxic CD8+ T cells recognize and kill Plasmodium vivax–infected reticulocytes." Nature medicine (2018): 1.

Whole blood aliquots

Pácal, Lukáš, et al. " Deleterious Effect of Advanced CKD on Glyoxalase System Activity not Limited to Diabetes Aetiology ." International Journal of Molecular Sciences 19.5 (2018): 1517.

Serum Samples

Dalby, M. J. (2017). The Role Of The Intestinal Microbiota In The Modulation Of Food Intake And Body Weight. OpenDalby, Matthew J.“THE ROLE OF THE INTESTINAL MICROBIOTA IN THE MODULATION OF FOOD INTAKE AND BODY WEIGHT”. Thesis Commons, 20.

Porcine D-dimer

Huang, S. (2017). The Effect of 120-kHz Ultrasound on Thrombolytic Efficacy in Porcine Thromboembolism Models (Doctoral dissertation, University of Cincinnati).

RBC perfused livers (Hepatic primary cell line cultures)

Laing, R. W., Bhogal, R. H., Wallace, L., Boteon, Y., Neil, D. A., Smith, A., ... & Afford, S. C. (2017). The use of an acellular oxygen carrier in a human liver model of normothermic machine perfusion. Transplantation, 101(11), 2746-2756.

Total hemocyte lysates

O'Connell, Grant C., et al. "Monocyte-Lymphocyte Cross-Communication Via Soluble CD163 Directly Links Innate Immune System Activation And Adaptive Immune System Suppression Following Ischemic Stroke." bioRxiv (2017): 144063.

Bronchoalveolar lavage fluid (BALF)

Maneesh Bhargava, Kevin J. Viken, Sanjoy Dey, Michael S. Steinbach, Baolin Wu, Pratik D. Jagtap, LeeAnn Higgins, Angela Panoskaltsis-Mortari, Daniel J. Weisdorf, Vipin Kumar, Mukta Arora, Peter B. Bitterman, David H. Ingbar, Chris H. Wendt. Proteome Profiling in Lung Injury after Hematopoietic Stem Cell Transplantation. Biology of Blood and Marrow Transplantation 22 (2016) 1383-1390.

Cell Lines

Phanse, Sadhna, Cuihong Wan, Blake Borgeson, Fan Tu, Kevin Drew, Greg Clark, Xuejian Xiong et al. "Proteome-wide dataset supporting the study of ancient metazoan macromolecular complexes." Data in brief 6 (2016): 715-721

Dried Blood Spot(DBS)/Whole Blood

Hakuna, Lovemore, et al. " A simple assay for glutathione in whole blood."Analyst (2015).

Whole Blood

Chalásová, Katarína, et al. "Transketolase Activity but not Thiamine Membrane Transport Change in Response to Hyperglycaemia and Kidney Dysfunction." Experimental and Clinical Endocrinology & Diabetes (2017).

Chalásová, Katarína. Functional analysis of genetic variants in genes associated with the development of glycotoxic damage. Diss. Masarykova univerzita, Lékařská fakulta, 2017.

Lahut, Suna, et al. "Blood RNA biomarkers in prodromal PARK4 and REM sleep behavior disorder show role of complexin-1 loss for risk of Parkinson's disease." Disease Models & Mechanisms (2017): dmm-028035.

Brittain, Matthew K., Kevin G. McGarry, Robert A. Moyer, Michael C. Babin, David A. Jett, Gennady E. Platoff, and David T. Yeung. "Efficacy of Recommended Prehospital Human Equivalent Doses of Atropine and Pralidoxime Against the Toxic Effects of Carbamate Poisoning in the Hartley Guinea Pig." International journal of toxicology (2016): 1091581816638086.

Snider, Thomas H., Kevin G. McGarry, Michael C. Babin, David A. Jett, Gennady E. Platoff Jr, and David T. Yeung. "Acute toxicity of phorate oxon by oral gavage in the Sprague-Dawley rat." Fundamental Toxicological Sciences 3, no. 5 (2016): 195-204.

Bhargava, Maneesh, Kevin J. Viken, Sanjoy Dey, Michael S. Steinbach, Baolin Wu, Pratik D. Jagtap, LeeAnn Higgins et al. "Proteome Profiling in Lung Injury after Hematopoietic Stem Cell Transplantation." Biology of Blood and Marrow Transplantation (2016).

Snider, Thomas H., Christina M. Wilhelm, Michael C. Babin, Gennady E. Platoff Jr, and David T. Yeung. "Assessing the therapeutic efficacy of oxime therapies against percutaneous organophosphorus pesticide and nerve agent challenges in the Hartley guinea pig."The Journal of Toxicological Sciences 40, no. 6 (2015): 759-775.

Blood Plasma

Johns, Michael, et al. "SR-135, a peroxynitrite decomposing catalyst, enhances β-cell function and survival in B6D2F1 mice fed a high fat diet."Archives of Biochemistry and Biophysics (2015).

Asleh, Rabea, et al. " Haptoglobin Genotype-dependent Differences in Macrophage Lysosomal Oxidative Injury." Journal of Biological Chemistry289.23 (2014): 16313-16325.

Biological Fluids

J Krupey - United States Patent: 10/180,053, 2002 Removal of extraneous substances from biological fluids containing nucleic acids and the recovery of nucleic acids

Lung Tissue Specimens & Pulmonary Research (Acute Respiratory Distress Syndrome & Acute Hypoxic Failure)

Bhargava, Maneesh""Proteomic Studies in Acute Hypoxic Respiratory Failure." PhD diss., UNIVERSITY OF MINNESOTA. (2015).

Red Blood Cell (RBC) - Lysates.

Kyoungsook Park, Christopher D. Saudek, and Gerald W. Hart Increased Expression of β-N-Acetylglucosamindase (O-GlcNAcase) in Erythrocytes from Prediabetic and Diabetic Individuals. Diabetes.2010;59(7):1845-50.

Barasa, Benjamin, and Monique Slijper. " Challenges for red blood cell biomarker discovery through proteomics." Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1844.5 (2014): 1003-1010

Stored Blood Products

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Red Blood Cells/Forensic Research

Danielson, Phillip B. "Isolation of Highly Specific Protein Markers for the Identification of Biological Stains: Adapting Comparative Proteomics to Forensics." (2011).