Highlightsfrom Biotech Support Group Exhibiting at World Human ProteomeCongress

BiotechSupport Group (BSG) exhibited at the 2025 World Human Proteome (HUPO)Congress, November 9-13, 2025 in Toronto Canada. NRicher™Beads – for targeted serum proteomic biomarker enrichment,simplified and diversified workflows. The NRicher™Knowledgebase, for bead enrichment of user-selected targets

New Jersey Commission On Science,Innovation And Technology Phase II Catalyst Research Grant Awarded toBiotech Support Group

BiotechSupport Group announces receipt of a NEW JERSEY COMMISSION ONSCIENCE, INNOVATION AND TECHNOLOGY Phase II Catalyst Research Awardof $40,000. TheResearch Award entitled “ Evaluatingthe NRicher™ Bead Platform for Targeted LC-MS Quantification” will be conducted at The Rutgers Biological Mass SpectrometryFacility. This award follows from the Catalyst Phase I Awarddemonstrating simple workflows that generate low abundance enrichmentof sub-proteomes suitable for quantitative plasma proteomics.

SwapnaLS, Stevens GC, Sardinha-Silva A, Hu LZ, Brand V, Fusca DD, et al.(2024 )ToxoNet: A high confidence map of protein-protein interactions inToxoplasma gondii. PLoSComput Biol 20(6):e1012208. https://doi.org/10.1371/ journal.pcbi.1012208

Thearticle states we used affinity beads (NuGel PROspector)to pre-enrich Toxoplasma gondii lysate to capture five distinctsubproteomes. [Note: NuGel PROspector beads are now part of theNRicher™ platform.]. When comparing the 5 differentsubproteomes, there is clearly different selection biases amongst the5 surface chemistries. Also, many of the proteins observed from theNRicher™ beads, were not observed in the Ion exchangefractions demonstrating the importance of combining different modes(ionic, hydrophobic, etc.) of separation to alter selectionproperties, and consequently improving overall proteome coverage.

Efficienciesgained in targeted serum proteomics using NRicher Beads –simplified and diversified workflows for sub-proteome and biomarkerenrichment – Poster HUPO World Congress 2024

AfterNRicher™ sample prep, target peptides have highly enhancedspectral signal. NRicher™ sub-proteome enrichment canminimize acquisition time, collectively improving overall throughput,cost, and productivity. Specific target peptides that reportfunctional PTMs and amino acid variant regions promise insights andpotential multiplex biomarkers for disease.

https://www.biotechsupportgroup.com/v/vspfiles/tem...

Wan,C., Borgeson, B., Phanse, S. et al. Panoramaof ancient metazoan macromolecular complexes.Nature 525, 339–344 (2015). hXps://doi.org/10.1038/nature14877

Sixdifferent NRicher™ beads (described with an old tradenamePROspector) were used as an enrichment step in the overall workflow;about twice the number of observations and annotations becamepossible. This further validates that the sub-proteome biascharacteristics of the NRicher™ surface chemistry platformcan simplify complex proteomes into enriched sub-proteomes withefficiencies suitable for deep functional proteomecharacterization.

Whitepaper- NRicher™: A Low Abundance Proteome Enrichment Platform WithSeamless Integration of On-Bead Digestion

TheNRicher™ Advantage is described: • Consumable chemically derivedNuGel™ beads, species agnostic as they are not derived fromantibodies • Does not require any specialized instruments, just astandard microfuge • Use of bead cocktails allows for one, ratherthan multiple LC-MS analyses • Functionally active sub-proteomesafter separations, for any orthogonal functional, enzymatic, orimmunoassay analysis

https://www.biotechsupportgroup.com/v/vspfiles/templates/257/pdf/BiotechSupportGroup-NRicher-Whitepaper.pdf

NRicher: Family Specific Enrichment For Targeted Proteomics – Poster USHUPO 2024

Theneed for new biomarkers to support personalized healthcare, hasfostered numerous proteomic innovations. Still, a number ofchallenges remain. One is the preponderance of high abundanceproteins and, concurrently in targeted proteomic workflows,efficiency and consistency in quantifying target peptides fromdifferent sample cohorts. This is in part due to the changinglandscape of proteins/peptides not associated with the selectedtargets. A solution for both these challenges is now availablethrough a suite of products called NRicher .

https://www.biotechsupportgroup.com/v/vspfiles/templates/257/pdf/NRicher%20poster%20small.pdf