Glutathione Peroxidase (GPX) Assay Kit

소개 (Description)

Glutathione peroxidase (GPX) is a family of antioxidant enzymes whose primary function is to protect cells against oxidative damage. These enzymes catalyze the reduction of various peroxide species, including hydrogen peroxide (H₂O₂) and lipid hydroperoxides (ROOH), using reduced glutathione (GSH) as an electron donor. Through this activity, GPX plays a crucial role in maintaining cellular redox homeostasis and preventing membrane lipid peroxidation.
BQC Glutathione Peroxidase Activity Assay Kit is based on a coupled reaction involving the enzyme glutathione reductase (GR) using NADPH as a cofactor. The oxidation of NADPH to NADP⁺ results in a decrease in absorbance at 340 nm, which is monitored spectrophotometrically.
This kit is a sensitive and reproducible assay to measure GPX activity in biological samples.

Glutathione Peroxidase (GPX) 원리

BQC Glutathione peroxidase Activity Assay Kit is based on a coupled reaction involving the enzyme glutathione reductase (GR). In the first reaction, GPX catalyzes the reduction of hydroperoxides (ROOH) generating oxidized glutathione (GSSG). Subsequently, the GSSG is reduced back to GSH by GR, using NADPH as a cofactor:

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